Triiodide derivatization in protein crystallography

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Triiodide derivatization in protein crystallography.

Methods for producing protein derivatives using cryosoak techniques with triiodide solutions are described. The methods have been tested using six different proteins. SAD/SIRAS phasing was attempted for each protein using data measured with conventional Cu Kalpha X-ray equipment and synchrotron radiation. Refinement of all six derivative structures showed that iodine is able to bind as I(-) (as...

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ژورنال

عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography

سال: 2003

ISSN: 0907-4449

DOI: 10.1107/s0907444903012897